duoset dy Search Results


duoset elisa development kit  (R&D Systems)
98
R&D Systems duoset elisa development kit
TNF-α release in triple cell co-cultures upon particle incubation. TNF-α levels in the supernatants (upper chamber, lower chamber) were measured by <t>ELISA.</t> TNF-α release in cells exposed to LPS, 1 μm, and 0.078 μm polystyrene particles, TiO 2 , and gold NP. Values are means ± SD of 3 experiments. * indicates a statistical difference to the levels in the supernatants in the control of the upper chamber, § indicates a statistical difference to the levels in the supernatants in the control of the lower chamber.
Duoset Elisa Development Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits  (R&D Systems)
99
R&D Systems elisa kits
TNF-α release in triple cell co-cultures upon particle incubation. TNF-α levels in the supernatants (upper chamber, lower chamber) were measured by <t>ELISA.</t> TNF-α release in cells exposed to LPS, 1 μm, and 0.078 μm polystyrene particles, TiO 2 , and gold NP. Values are means ± SD of 3 experiments. * indicates a statistical difference to the levels in the supernatants in the control of the upper chamber, § indicates a statistical difference to the levels in the supernatants in the control of the lower chamber.
Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sandwich duoset elisa development systems  (R&D Systems)
90
R&D Systems sandwich duoset elisa development systems
TNF-α release in triple cell co-cultures upon particle incubation. TNF-α levels in the supernatants (upper chamber, lower chamber) were measured by <t>ELISA.</t> TNF-α release in cells exposed to LPS, 1 μm, and 0.078 μm polystyrene particles, TiO 2 , and gold NP. Values are means ± SD of 3 experiments. * indicates a statistical difference to the levels in the supernatants in the control of the upper chamber, § indicates a statistical difference to the levels in the supernatants in the control of the lower chamber.
Sandwich Duoset Elisa Development Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa set  (R&D Systems)
94
R&D Systems elisa set
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Elisa Set, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human neurotrophin 3 duoset elisa immunoassay kit  (R&D Systems)
93
R&D Systems human neurotrophin 3 duoset elisa immunoassay kit
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Human Neurotrophin 3 Duoset Elisa Immunoassay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 30  (R&D Systems)
93
R&D Systems il 30
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Il 30, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunosorbent assay elisa  (R&D Systems)
94
R&D Systems immunosorbent assay elisa
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Immunosorbent Assay Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human il 17f elisa set  (R&D Systems)
94
R&D Systems human il 17f elisa set
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Human Il 17f Elisa Set, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse il 1β il 1f2 duoset elisa  (R&D Systems)
96
R&D Systems mouse il 1β il 1f2 duoset elisa
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Mouse Il 1β Il 1f2 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ccl2 mcp 1 duoset elisa  (R&D Systems)
96
R&D Systems human ccl2 mcp 1 duoset elisa
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Human Ccl2 Mcp 1 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tnf α  (R&D Systems)
99
R&D Systems tnf α
Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation <t>of</t> <t>CXCL1</t> expression in p53 ko LOX-IMVI and SK-MEL-5 cells by <t>Elisa</t> analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).
Tnf α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TNF-α release in triple cell co-cultures upon particle incubation. TNF-α levels in the supernatants (upper chamber, lower chamber) were measured by ELISA. TNF-α release in cells exposed to LPS, 1 μm, and 0.078 μm polystyrene particles, TiO 2 , and gold NP. Values are means ± SD of 3 experiments. * indicates a statistical difference to the levels in the supernatants in the control of the upper chamber, § indicates a statistical difference to the levels in the supernatants in the control of the lower chamber.

Journal: Particle and Fibre Toxicology

Article Title: Translocation of particles and inflammatory responses after exposure to fine particles and nanoparticles in an epithelial airway model

doi: 10.1186/1743-8977-4-9

Figure Lengend Snippet: TNF-α release in triple cell co-cultures upon particle incubation. TNF-α levels in the supernatants (upper chamber, lower chamber) were measured by ELISA. TNF-α release in cells exposed to LPS, 1 μm, and 0.078 μm polystyrene particles, TiO 2 , and gold NP. Values are means ± SD of 3 experiments. * indicates a statistical difference to the levels in the supernatants in the control of the upper chamber, § indicates a statistical difference to the levels in the supernatants in the control of the lower chamber.

Article Snippet: After centrifugation, TNF-α was quantified by a commercially available DuoSet ELISA Development kit (R&D Systems, Catalogue Number: DY 210, Oxon, UK) according to the manufacturer's recommendations.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Control

Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation of CXCL1 expression in p53 ko LOX-IMVI and SK-MEL-5 cells by Elisa analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).

Journal: Cells

Article Title: p53 Promotes Cytokine Expression in Melanoma to Regulate Drug Resistance and Migration

doi: 10.3390/cells11030405

Figure Lengend Snippet: Reduced cytokine secretion in p53 knockout melanoma lines. ( A ) Comparative analysis of ligands abundance in supernatant from p53 ko and p53 WT LOX-IMVI cells by multiplex cytokine array. Differential expression of secreted ligand for each knockout clone compared to wild type is shown in the heatmap (as in log2-fold change). The observed ligand concentration (pg/mL) in p53 WT cells are depicted on the right side by numbers >10, 1–10, and <1. Cytokines analyzed by qPCR (in E and E) are indicated by an asterisk on the left side. ( B ) Expression of a subset of the ligands in wild-type and knockout clones by multiplex analysis is demonstrated. n = 3 (mean ± S.D), except for LIF, in which two biological samples had shown the value below the detection limit. ( C ) Validation of CXCL1 expression in p53 ko LOX-IMVI and SK-MEL-5 cells by Elisa analysis; n = 3 (mean ± S.D). Significance was determined by t -test in comparison to the wild-type group, and the observed level of significance is shown by asterisk (* < 0.05; ** < 0.005; *** < 0.0005; **** < 0.0001).

Article Snippet: Human CXCL1 expression was analyzed using Elisa set (R & D system, DY 275), according to company instruction.

Techniques: Knock-Out, Multiplex Assay, Quantitative Proteomics, Concentration Assay, Expressing, Clone Assay, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Comparison